Methods & compositions for improving protein production
AssigneeVentria Bioscience
InventorsMichael E. Barnett, Matthew S. Croughan
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Abstract
The invention relates to compositions, and uses thereof, which are beneficial for eukaryotic cells in culture, and methods for their use in promoting cell growth, viability and recombinant protein expression. The methods disclosed in the present application are useful, for example, for improving cell viability and in accelerating the rate of cell growth of cells grown in culture. In one aspect, the supplements of the invention are useful for improving or enhancing the yield of the recombinant proteins from the cell cultures.
Claims16
1-72. (canceled)
A method for enhancing the growth of a cell growing in cell culture medium, comprising adding a supplement comprising recombinant albumin to said cell culture medium,
- wherein said recombinant albumin is produced in a plant;
- wherein said supplement has less than about 1 EU of endotoxin per mg of albumin;
- and
- wherein said albumin comprises less than about 2% aggregated albumin.
A method for enhancing the productivity of a cell that has been adapted to serum free media, comprising adding a supplement comprising recombinant albumin to said serum free media,
- wherein said recombinant albumin is produced in a plant;
- wherein said supplement has less than about 1 EU of endotoxin per mg of albumin;
- and
- wherein said albumin comprises less than about 2% aggregated albumin.
Dependencies are inferred from the claim text.