Cell-culture-bag
AssigneeXpand Biotechnology B.V.
InventorsElise Leonore Isolde Dijkhuizen Borgart, Madelon Sophia George Maria Bracke, Joost Dick De Bruijn
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Abstract
A cell-culture-bag for use in the expansion of stem cells from a crude biopsy, comprising: outer walls; a chamber located within the walls; a first inlet, and first and second outlets, providing fluid communication with the chamber, for connection to a perfusion apparatus; and a filter arrangement constructed to allow passage of red blood cells and block passage of stem cells from the first outlet, and block passage of microcarriers from the second outlet.
Claims18
A cell-culture-bag for use in the expansion of stem cells from a crude biopsy, comprising:
- outer walls;
- a chamber located within the walls;
- a first inlet, and first and second outlets, providing fluid communication with the chamber, for connection to a perfusion apparatus;
- and
- a filter arrangement constructed to allow passage of red blood cells and block passage of stem cells from the first outlet, and block passage of microcarriers from the second outlet.
A system for expanding cells from a crude biopsy, comprising:
- a cell-culture-bag comprising a chamber;
- a first inlet, and a seeding/expansion outlet, and a collection outlet, each in fluid communication with the chamber;
- a perfusion apparatus connected to the first inlet, and the seeding/expansion and collection outlets;
- wherein the perfusion apparatus has a seeding mode in which the first inlet and the seeding/expansion outlet are in operative connection with a seeding circuit of the perfusion apparatus, and a seeding operation is performed on stem cells within the chamber;
- wherein the perfusion apparatus has an expansion mode in which the first inlet and the seeding/expansion outlet are in operative connection with an expansion circuit of the perfusion apparatus, and an expansion operation is performed on stem cells within the chamber;
- wherein the perfusion apparatus has a harvesting mode in which the first inlet is in operative connection with a harvesting section of the perfusion apparatus, and an harvesting operation is performed on stem cells within the chamber;
- wherein the perfusion apparatus has a collection mode in which the collection outlet is in operative connection with a collection section of the perfusion apparatus, and harvested stem cells exit from the collection outlet.
Dependencies are inferred from the claim text.