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AssigneeChlor Bioenergy Inc.
InventorsFan Lu, Zhongyang Deng
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A process for production of oil-rich microalgae is disclosed, which includes methods for cultivating strains of Characium polymorphum and/or Ankistrodesmus braunii (Chlorophyceae), or isolated variants thereof, in order to produce oil at optimum levels. The process is suitable for large-scale productions. The invention also discloses methods for purifying the microalgae cells, and methods for treating the microalgae cells to enrich their oil content. In addition, various representative culturing media as well as conditions for cultivating microalgae and inducing oil accumulation are also disclosed. The oil-rich microalgae produced by the process can be used as feedstock for biofuel production.
A process for production of oil-rich microalgae, the process comprising:
A culture medium for enhancing oil accumulation in microalgae, comprising about 0.05-1.75 g/L MgSO4, about 0.5-3.6 g/L Na2CO3, about 0.05-0.2 g/L CaCl2, about 0.001 g/L EDTA, about 0.02-1.2 g/L K2HPO4, about 0.006 g/L Citric Acid, about 0.006 g/L Fe(NH4)2Citric, and about 0.2-1 ml/liter A5 micronutrients.
Use of cells or strains of Characium polymorphum, Ankistrodesmus braunii, or isolated varieties or combinations thereof, in production of oil-rich microalgae useful as feedstock for production of biodiesel.
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Dependencies are inferred from the claim text.