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US 2013/0157342 A1Published application
Novel aldolase and production process of substituted alpha-keto acids
AssigneeAjinomoto Co., Inc.
InventorsMasakazu SUGIYAMA, Kunihiko Watanabe, Shigeru Kawahara
Life of this patent
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Abstract
4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.
Claims11
An isolated polynucleotide of following (a) or (b):
- (e) a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 15 or the nucleotide sequence of nucleotides 398 to 1141 of SEQ ID NO:
- 15;
- or
- (f) a polynucleotide that hybridizes under stringent conditions with a polynucleotide having the nucleotide sequence complementary to the full-length nucleotide sequence of SEQ ID NO:
- 15 or the nucleotide sequence of nucleotides 398 to 1141 of SEQ ID NO:
- 15, and encodes a protein having aldolase activity, wherein said stringent conditions comprise a wash step in 0.1×SSC and 0.1% SDS at 65° C.
4 more elements
An isolated polynucleotide of following (c) or (d):
- (g) a polynucleotide that encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 16;
- or
- (h) a polynucleotide that encodes a protein having an amino acid sequence that contains a substitution, deletion, insertion, or addition of one to ten amino acid residues in the amino acid sequence of SEQ ID NO:
- 16, and has aldolase activity.
2 more elements
A recombinant DNA produced by ligating into a vector DNA a polynucleotide selected from the group consisting of:
- (e) a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 15 or the nucleotide sequence of nucleotides 398 to 1141 of SEQ ID NO:
- 15;
- (f) a polynucleotide that hybridizes under stringent conditions with a polynucleotide having the nucleotide sequence complementary to the full-length nucleotide sequence of SEQ ID NO:
- 15 or the nucleotide sequence of nucleotides 398 to 1141 of SEQ ID NO:
- 15, and encodes a protein having aldolase activity, wherein said stringent conditions comprise a wash step in 0.1×SSC and 0.1% SDS at 65° C.;
- (g) a polynucleotide that encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 16;
- and
- (h) a polynucleotide that encodes a protein having an amino acid sequence that contains a substitution, deletion, insertion, or addition of one to ten amino acid residues in the amino acid sequence of SEQ ID NO:
- 16, and has aldolase activity.
8 more elements
Dependencies are inferred from the claim text.