L-malate production by metabolically engineered escherichia coli
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsXueli Zhang, Xuan Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Abstract
A process for the production of malic acid in commercially significant quantities from the carbon compounds by genetically modified bacterial strains (GMBS; also referred to as biocatalysts or genetically modified microorganisms) is disclosed. Microorganisms suitable for the production of malic acid can be cultured in one or two-step processes as disclosed herein.
Claims21
1-69. (canceled)
An isolated bacterial cell, said bacterial cell lacking fumarate reductase (FRD) activity and having increased levels of phosphoenol pyruvate carboxykinase (PCK) activity.
An isolated bacterial cell, said bacterial cell lacking fumarase (FUM) activity and having increased levels of phosphoenol pyruvated carboxykinase (PCK) activity.
Dependencies are inferred from the claim text.