Loading patent…
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Hiromi Kambe, Masakazu Ito
Estimating the term…
A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.
1-20. (canceled)
A gene encoding the mutant hydroxyalkanoic acid synthase, wherein the proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis comprising the amino acid sequence as shown in SEQ ID NO:
A gene encoding the mutant hydroxyalkanoic acid synthase, wherein the valine residue at position 131 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis comprising the amino acid sequence as shown in SEQ ID NO:
A gene encoding the mutant hydroxyalkanoic acid synthase, wherein the histidine residue at position 17 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis comprising the amino acid sequence as shown in SEQ ID NO:
A gene encoding the mutant hydroxyalkanoic acid synthase, wherein the leucine residue at position 230 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis comprising the amino acid sequence as shown in SEQ ID NO:
Dependencies are inferred from the claim text.