Method for Simultaneous Fermentation of Pentose and Hexose
AssigneeFeng Chia University
InventorsYUN-PENG CHAO, CHUNG-JEN CHIANG, HONG-MIN LEE, ZEI-WEN WANG, PO-TING CHEN
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Abstract
The present invention relates to a method for simultaneous fermentation of pentose and hexose. The present invention modifies the metabolic pathways of a target microorganism in order to enable the target microorganism to rapidly metabolize pentose and hexose at the same time. This present invention simplified the fermentation process, decreased the cost, and increased the efficiency of the fermentation process.
Claims28
A method enabling a microorganism to ferment pentose and hexose simultaneously, which method comprises steps of:
- (a) deleting a gene sequence of glucose permease in a target microorganism;
- (b) introducing a glucose facilitator gene sequence into the target microorganism;
- (c) introducing at least one promoter into upstream of at least one of the gene sequence in pentose phosphate pathway of the target microorganism;
- and
- (d) deleting at least one of gene sequence responsible for synthesis of organic acid in the target microorganism.
A method enabling a microorganism to ferment pentose and hexose simultaneously comprises following steps:
- (a) deleting a ptsG gene sequence in a target microorganism;
- (b) introducing a glf gene sequence into the target microorganism;
- (c) introducing a first promoter into upstream of a rpe and a tktA gene sequences in the target microorganism;
- (d) introducing a second promoter into upstream of a rpiA and a talB gene sequences in the target microorganism;
- (e) deleting a poxB gene sequence of the target microorganism;
- (f) deleting a pta gene sequence of the target microorganism;
- (g) deleting a ldhA gene sequence of the target microorganism;
- and
- (h) deleting a frdA gene sequence of the target microorganism.
Dependencies are inferred from the claim text.