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US 2013/0157311 A1Published application
Methods of Determination of Activation or inactivation of Atrial Natriuretic Peptide (ANP) and Brain Natriuretic Peptide (BNP) Hormonal Systems
AssigneeOrion Diagnostica Oy
InventorsOlli Vuolteenaho, Minna Ala-Kopsala, Heikki Ruskoaho, Juhani Leppäluoto, Jouko Haapalahti
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Abstract
An in vivo method of determining activation or inactivation of the atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) hormonal systems, the method comprising simultaneously detecting the presence or amount of atrial and brain natriuretic peptide prohormones (proANP and proBNP) or fragments thereof in a sample.
Claims14
An agent which comprises:
- (a) (i) proANP (SEQ ID NO:1), ANP (SEQ ID NO:2), or NT-proANP (SEQ ID NO:3);
- (ii) a homologous sequence having at least 70% identity to (a)(i);
- or
- (iii) a fragment of (a)(i) or (a)(ii) which is at least 6 amino acids in length;
- and
- (b) (i) pro-BNP (SEQ ID NO:4), BNP (SEQ ID NO:5), or NT-proBNP (SEQ ID NO:6);
- (ii) a homologous sequence having at least 70% identity to (b)(i);
- or
- (iii) a fragment of (b)(i) or (b)(ii) which is at least 6 amino acids in length.
6 more elements
A diagnostic kit comprising:
- (a) a bi or oligo specific first binding substance that is able to bind to both
- (I) (i) proANP (SEQ ID NO:
- 1), ANP (SEQ ID NO:
- 2), or NT-proANP (SEQ ID NO:
- 3);
- (ii) a homologous sequence having at least 70% identity to (i);
- or
- (iii) a fragment of (i) or (ii) which is at least 6 amino acids in length;
- and
- (II) (i) proBNP (SEQ ID NO:
- 4), BNP (SEQ ID NO:
- 5), or NT-proBNP (SEQ ID NO:
- 6);
- (ii) a homologous sequence having at least 70% identity to (i);
- or
- (iii) a fragment of (i) or (ii) which is at least 6 amino acids in length;
- or
- (b) said first binding substance and a fusion polypeptide agent comprising both:
- (I) proANP (SEQ ID NO:1), ANP (SEQ ID NO:2), or NT-proANP (SEQ ID NO:3);
- and
- (II) proBNP (SEQ ID NO:4), BNP (SEQ ID NO:5), or NT-proBNP (SEQ ID NO:6), wherein said fusion polypeptide agent can be bound by said first binding substance, and optionally the binding substance and/or the agent is labelled.
18 more elements
Dependencies are inferred from the claim text.