Method for Production and Use of Mite Group 1 Proteins

A method to produce a recombinant protein comprising a recombinant mite Group 1 protein or a fragment thereof, comprising the steps of:

• (a) culturing a methyltrophic yeast microorganism transformed with a nucleic acid molecule encoding said recombinant mite Group 1 protein or fragment;
• and
• (b) recovering said recombinant mite Group 1 protein or fragment from said methyltrophic yeast microorganism;
• wherein
• said recombinant mite Group 1 protein or fragment is selected from the group consisting of:
• Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Euroglyphus maynei recombinant mite Group 1 proteins (Der f 1, Derp 1 and Eur m 1) or a fragment thereof and has a function selected from the group consisting of:
• selectively binding IgE and causing proliferation of a T cell that proliferates in response to a native mite Group 1 protein selected from the group consisting of Der f 1, Der p 1 and Eur m 1 and
• said nucleic acid molecule hybridizes under stringent conditions with a nucleic acid sequence selected from the group consisting of:
• SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:22, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:31, SEQ ID NO:34, SEQ ID NO:37, SEQ ID NO:40, SEQ ID NO:43, SEQ ID NO:64, SEQ ID NO:69, SEQ ID NO:76, SEQ ID NO:81, SEQ ID NO:86, and SEQ ID NO:91 or a fragment thereof and wherein said nucleic acid molecule is optionally fused to a signal sequence.

A method to produce a recombinant protein comprising a recombinant mite Group 1 protein or a fragment thereof, comprising the steps of:

• (a) culturing a methyltrophic yeast microorganism transformed with a nucleic acid molecule encoding said recombinant mite Group 1 protein or fragment;
• and
• (b) recovering said recombinant mite Group 1 protein or fragment from said methyltrophic yeast microorganism;
• wherein
• said recombinant mite Group 1 protein or fragment is selected from the group consisting of:
• Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Euroglyphus maynei recombinant mite Group 1 proteins (Der f 1, Der p 1 and Eur m 1) or a fragment thereof and has a function selected from the group consisting of:
• selectively binding IgE and causing proliferation of a T cell that proliferates in response to a native mite Group 1 protein selected from the group consisting of Der f 1, Der p 1 and Eur m 1 and
• said nucleic acid molecule having a sequence identity of at least 95% with a nucleic acid sequence selected from the group consisting of:
• SEQ ID NO:
• 1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:19, SEQ ID NO:22, SEQ ID NO:25, SEQ ID NO:28, SEQ ID NO:31, SEQ ID NO:34, SEQ ID NO:37, SEQ ID NO:40, SEQ ID NO:43, SEQ ID NO:64, SEQ ID NO:69, SEQ ID NO:76, SEQ ID NO:81, SEQ ID NO:86, and SEQ ID NO:91 or a fragment thereof and wherein said nucleic acid molecule is optionally fused to a signal sequence.