Methods of obtaining genetic competence in bacillus cells
AssigneeNovozymes, Inc.
InventorsRandy Berka, Michelle Maranta, Maria Tang, Barbara Cherry
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Abstract
The present invention relates to methods of obtaining genetic competence in non-competent Bacillus cells for their transformation with exogenous DNA.
Claims17
A method of obtaining a Bacillus licheniformis transformant, comprising:
- (a) transforming an exogenous DNA into a Bacillus licheniformis host cell made competent by at least one copy of an introduced first nucleic acid construct comprising a promoter region operably linked to a polynucleotide encoding a ComS polypeptide, wherein the polynucleotide encoding the ComS polypeptide is foreign to the Bacillus licheniformis host cell that was non-competent prior to introduction of the first nucleic acid construct;
- and
- (b) isolating a transformant of the Bacillus licheniformis host cell comprising the DNA.
A method of obtaining a competent Bacillus licheniformis host cell, comprising:
- (a) introducing into a non-competent Bacillus licheniformis host cell at least one copy of a first nucleic acid construct comprising a promoter region operably linked to a polynucleotide encoding a ComS polypeptide, wherein the polynucleotide encoding the ComS polypeptide is foreign to the Bacillus licheniformis host cell;
- and
- (b) isolating a competent Bacillus licheniformis host cell comprising the polynucleotide encoding the ComS polypeptide.
A competent Bacillus licheniformis host cell comprising at least one copy of an introduced first nucleic acid construct comprising a promoter region operably linked to a polynucleotide encoding a ComS polypeptide, wherein the polynucleotide encoding the ComS polypeptide is foreign to the Bacillus licheniformis host cell that was non-competent prior to introduction of the first nucleic acid construct.
Dependencies are inferred from the claim text.