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AssigneeUniversity of South Carolina
InventorsDavid Reisman, Amanda Polson
Estimating the term…
A bidirectional expression vector is described that can be utilized to determine the existence and characteristics of bidirectional promoters. The bidirectional expression vector includes two different reporter genes in a head to head (5′ to 5′) arrangement. In addition, the bidirectional expression vector can include a polylinker region located between the heads of the two reporter genes that provides multiple cloning sites for nonexclusive examination of polynucleotide sequences. The vector can also include a splicing site and drug resistance. The bidirectional expression vector can be used to examine a polynucleotide sequence for the presence of divergent regulator regions and, following determination of a bidirectional promoter, can be utilized to further elucidate characteristics of the bidirectional promoter.
A bidirectional expression vector comprising a first reporter gene and a second reporter gene, the first and second reporter genes being arranged in a head to head (5′ to 5′) arrangement, the bidirectional expression vector including a polylinker region between the heads of the first and second reporter genes, the bidirectional expression vector including multiple cloning sites within the polylinker region.
A method for the production of a first expression product of a first reporter gene and a second expression product of a second reporter gene from a bidirectional vector, the method including:
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