Method and system for evaluation of grafts
AssigneeTerumo Kabushiki Kaisha
InventorKenta SAKAMOTO
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Abstract
A method and system for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation are provided. In one form, the method includes determining multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation.
Claims13
A method for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation, the method comprising:
- determination of multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation,
- wherein the determination of the multinucleating ability comprises:
- (i) a step of dissociating a portion of one or not less than two graft samples into individual cells;
- (ii) a step of incubating the dissociated cells obtained in step (i);
- and
- (iii) a step of detecting multinucleate cells in a cell culture obtained upon the incubation in step (ii), and
- the thus determined multinucleating ability is compared with a preset threshold value.
A system for evaluation of competence for transplantation of a graft sample containing cells capable of multinucleation, the system comprising:
- a detecting unit which detects multinucleate cells;
- and
- a calculating unit which calculates the multinucleating ability.
A method for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation, the method comprising:
- determination of multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation,
- wherein the multinucleating ability is rate of multinucleation.
A method for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation, the method comprising:
- determination of multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation,
- wherein the graft sample is determined as competent for transplantation to a living body where the cells capable of multinucleation are skeletal myoblasts and the rate of multinucleation as the multinucleating ability to be determined is higher than a preset threshold value.
Dependencies are inferred from the claim text.