US 2013/0157288 A1Published application

Immobilised-bead immunomultiplex assay

AssigneeBima Limited

InventorsStephen Kilfeather, Alberto Taurozzi, Paul Lehmann, Elaine Linglee

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Abstract

Embodiments of this invention include image-based systems and methods for detection of one or more analytes. A surface has identifiable analyte-specific capture particle(s) immobilised thereto at any point of an assay, to which different analytes attach due to the affinity of analyte-specific capture molecule(s) linked to the surface of the capture particle(s) for the analyte. Analyte-specific detector molecules with conjugated detection moieties are then attached to the analyte, and a computer assisted, image-based detection system captures images of the capture particles with or without attached analytes and detector molecules. By using different subsets of analyte-specific capture molecules, each subset having a characteristic identifiable feature; it is now possible to perform capture particle-based, rapid multiplex assays of biological and non-biological analytes without flow. These image-based systems can be used to aid in diagnosis of disease, evaluation of therapy for disease, or laboratory investigation.

A non-flow based multi-analyte detection system not requiring remobilisation of capture particles, comprising:

(1) a first type of analyte-specific capture particle permanently immobilised on a detection surface, said capture particle having:
(i) at least one capture particle identification parameter that distinguishes said first type of said analyte-specific capture particle from another type of capture particle,
(ii) said first type of analyte-specific capture molecule having a binding moiety specific for a first analyte, said binding moiety attached to said capture particle;
(2) a first type of analyte-specific detector molecules, each of said detector molecules having:
(i) an analyte-specific binding moiety;
and
(ii) a luminescent moiety having an identification parameter different from that of said first type of capture particle;
(3) a second type of analyte-specific capture particle immobilised said detection surface, said second type of capture particle having:
(i) at least one capture particle identification parameter that is different from the capture particle identification parameter of said first type of analyte-specific capture particle;
(ii) said second type of analyte-specific capture particle having a binding moiety specific for a second analyte, said binding moiety bound to said capture particle, where
(4) upon binding of a first analyte to said first type of analyte-specific capture particle, upon binding of said first type of analyte-specific detector molecule to said first analyte bound to said first type of analyte-specific capture molecule, and upon binding of a second analyte to said second type of analyte-specific capture particle, and upon binding of said second type of analyte-specific detector molecule to said second analyte bound to said second type of analyte-specific capture molecule, forming:
a first complex of said first type of analyte-specific detector molecule, said first analyte, and said first type of analyte-specific capture particle immobilised on said detection surface and a second complex of said second type of analye-specific detector molecule, said second analyte, and said second type of analyte-specific capture particle molecule immobilised on said detection surface;
and
(5) a light source, and a camera operably linked to a computer.

11 more elements

Facts

Application

13/580,083Filed 4 Mar 2011

Priority

GB 1002785.218 Feb 2010

Published

20 Jun 2013

Term ends

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Classification11 codes

G01N 21/75
Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light: systems…
G01N 21/6428
Fluorescence; Phosphorescence: measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g.…
G01N 33/54306
With an insoluble carrier for immobilising immunochemicals: solid-phase reaction mechanisms
G01N 33/54313
With an insoluble carrier for immobilising immunochemicals: the carrier being characterised by its particulate form

Abstract

Claims34