Colorimetric and fluorescent proteins
AssigneeBoard of Trustees of Michigan State University
InventorsBabak Borhan, James Geiger, Wenjing Wang, Chrysoula Vasileiou, Kin Sing Lee, Tetyana Berbasova
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Abstract
The invention relates to intracellular lipid binding proteins that bind retinoids and/or dye ligands and that are modified to transmit or emit light at a variety of different wavelengths.
Claims57
An isolated nucleic acid encoding a modified polypeptide selected from a member of the intracellular lipid binding protein family, wherein the modified polypeptide transmits or emits light when bound to a retinoid or fluorescent dye molecule, and wherein the intracellular lipid binding protein has been modified so that an amino acid at any of positions 102-135 can form a Schiff base with a retinoid.
A modified polypeptide selected from a member of the intracellular lipid binding protein family, wherein the modified polypeptide transmits or emits light when bound to a retinoid or fluorescent dye molecule, and wherein the intracellular lipid binding protein has been modified so that an amino acid at any of positions 102-135 can form a Schiff base with a retinoid.
Dependencies are inferred from the claim text.