Plant farnesyltransferases
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsGuo-Hua Miao, Rebecca E Cahoon, Timothy George Helentjaris
Life of this patent
Estimating the term…
Abstract
This invention relates to an isolated nucleic acid fragment encoding a farnesyltransferase subunit. The invention also relates to the construction of a chimeric gene encoding all or a portion of the farnesyltransferase subunit, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the farnesyltransferase subunit in a transformed host cell.
Claims10
1-14. (canceled)
A method of selecting an isolated polynucleotide that reduces the level of expression of a farnesyltransferase beta subunit polypeptide in a maize cell, the method comprising the steps of:
- (a) constructing a recombinant DNA construct comprising a promoter operably linked to an isolated polynucleotide comprising all or a fragment of SEQ ID NO:11, wherein the fragment comprises a nucleotide sequence of at least 30 contiguous nucleotides of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17 and the NO:11;
- (b) introducing the recombinant DNA construct into a maize cell;
- (c) measuring the level of farnesyltransferase beta subunit polypeptide in the maize cell containing the recombinant DNA construct;
- (d) comparing the level of farnesyltransferase beta subunit polypeptide in the maize cell containing the recombinant DNA construct with the level of farnesyltransferase beta subunit polypeptide in a maize cell that does not contain the recombinant DNA construct;
- and
- (e) selecting the isolated polynucleotide that reduces the level of expression of the farnesvltransferase beta subunit polypeptide in the maize cell.
17-31. (canceled)
A method of selecting an isolated polynucleotide that reduces the level of expression of a farnesyltransferase beta subunit polypeptide in a maize plant, the method comprising the steps of:
- (a) constructing a recombinant DNA construct comprising a promoter operably linked to an isolated polynucleotide comprising all or a fragment of SEQ ID NO:11, wherein the fragment comprises a nucleotide sequence of at least 30 contiguous nucleotides of SEQ ID NO:11;
- (b) transforming a maize cell with the recombinant DNA construct;
- (c) regenerating a maize plant from the transformed maize cell of (b), wherein the maize plant comprises the recombinant DNA construct;
- (d) measuring the level of farnesyltransferase beta subunit polypeptide in the maize plant comprising the recombinant DNA construct;
- and
- (e) comparing the level of farnesyltransferase beta subunit polypeptide in the maize plant comprising the recombinant DNA construct with the level of farnesyltransferase beta subunit polypeptide in a maize plant that does not comprise the recombinant DNA construct;
- and
- (f) selecting the isolated polynucleotide that reduces the level of expression of the farnesyltransferase beta subunit polypeptide in the maize plant.
Dependencies are inferred from the claim text.