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US 2013/0157278 A1Published application
Setting of multiple priming oligonucleotides for solid gel amplification in hydrogels
AssigneeThe Governors of the University of Alberta
InventorAlexey Atrazhev
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Abstract
The present invention provides for a novel system and method for amplification and detection of nucleic acids within a microfluidic device wherein multiple nucleotides capable of priming PCR are present within the system and substantially sequestered within separate hydrogel posts therein.
Claims4
A method for detecting a nucleic acid molecule within a hydrogel post array comprising
- a) Depositing on a substantially planar surface at least one aqueous solution containing at least one nucleic acid capable of initiating an amplification of a selected template nucleic acid and a viscosity increasing solute, said at least one aqueous solutions not being in fluid communication with each other and forming a multiplicity of deposits;
- b) Allowing said aqueous solution to evaporate in said deposits;
- c) Establishing a hydrogel post array of 2×1 or greater containing a cell-free, enzymatic, nucleic-acid amplification system, said hydrogel posts comprising the array arranged so as any given post impinges upon only one deposit;
- d) distributing on at least one of said hydrogel posts nucleic acid molecules, at least one of which may comprise a template nucleic acid for said amplification system;
- e) incubating said hydrogel posts under conditions promoting the synthesis of an amplified nucleic acid product by said amplification system from said at least one template;
- wherein said amplification system comprises at least two non-immobilized nucleic acids capable of promoting synthesis of amplified nucleic acid product from said template.
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Dependencies are inferred from the claim text.