Device for capture and lysis of microorganisms from liquids and methods of use thereof

A device for capture and lysis of microorganisms from a liquid comprising:

• a) a filter housing consisting of a hollow body defining a fluid path, having an upper input end adapted for receiving a liquid, a lower outflow end, and a filter cavity therebetween;
• b) a filter, having an input face and an output face, secured within the filter cavity in an orientation perpendicular to the fluid path such that fluid entering the input end of the housing passes through the filter before exiting the output end, wherein the filter divides the filter cavity into an upper input cavity between the input end of the housing and the input face of the filter, and a lower ouput cavity between the output face of the filter and the output end of the housing;
• c) at least one bead disposed within the input cavity, wherein the bead can move freely within the input cavity when the device is agitated;
• d) a first sealing means for removably sealing the input end;
• and
• e) a second sealing means for removably sealing the output end.