Device and method for conducting direct quantitive real time pcr
AssigneeAlphahelix Molecular Diagnostics AB
InventorsLars Edvinsson, Magnus Molin
Life of this patent
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Abstract
A method and device for performing direct quantitative real time PCR in a crude sample (200), wherein said sample is subjected to a centrifugal force sufficient to separate components of the sample into a supernatant and a pellet, and wherein at least one light source (204) and at least one detector (205) are positioned so that the excitation light impinges on the sample in a position above said pellet, and said detector detects light emitted from a position above said pellet.
Claims22
A device for performing direct quantitative real time PCR, said device comprising a rotor for receiving one or more elongate containers having an opening for receiving one or more samples, a distal end, and a longitudinal axis, rotating said containers along a circular path and subjecting said samples in said containers to a centrifugal force, at least one light source for subjecting the sample to excitation light, and at least one detector for detection light emitted from the sample, wherein said rotor holds said elongate containers in a position where the longitudinal axis of each container is parallel to the radius of said rotor;
- at least one light source and said at least one detector are positioned in relation to the rotor so that the excitation light impinges on the container in a position between the distal end of the container and the opening of the container, and said detector detects light emitted from the sample from a position between the distal end of the container and the opening of the container.
(canceled)
A method for performing direct quantitative real time PCR on a sample comprising the steps of
- placing said sample in an elongate container,
- subjecting said sample in said container to a centrifugal force,
- subjecting the sample to excitation light from a light source,
- detecting light emitted from the sample using a detector, wherein said elongate containers are subjected to a centrifugal force in a rotor which holds the containers in a position where the longitudinal axis of each container is parallel to the radius of said rotor;
- wherein at least one light source and said at least one detector are positioned in relation to the rotor so that the excitation light impinges on the container in a position between the distal end of the container and the opening of the container, and said detector detects light emitted from the sample from a position between the distal end of the container and the opening of the container.
(canceled)
A method for performing direct quantitative real time PCR on a sample, comprising:
- placing said sample in an elongate container,
- subjecting said sample in said container to a centrifugal force,
- subjecting the sample to excitation light from a light source,
- detecting light emitted from the sample using a detector,
- wherein said elongate containers are subjected to a centrifugal force in a rotor which holds the containers in a position where the longitudinal axis of each container is parallel to the radius of said rotor;
- wherein at least one light source and said at least one detector are positioned in relation to the rotor so that the excitation light impinges on the container in a position between the distal end of the container and the opening of the container, and said detector detects light emitted from the sample from a position between the distal end of the container and the opening of the container, and
- wherein the interface between the pellet and the supernatant is detected, and said at least one light source and/or said at least one detector are moved in relation to the rotor so that the excitation light impinges on the container in a position above the pellet formed in the container, and said detector detects light emitted from the sample from a position above the pellet formed in the container.
Dependencies are inferred from the claim text.