Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
AssigneeInterpace Diagnostics Corporation
InventorsAlex Adai, Anna Szafranska-Schwarzbach, Bernard Andruss, Stephan Albrecht Hahn
Life of this patent
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Abstract
Provided are methods and compositions for identifying a miRNA profile for a particular condition, such as pancreatic disease, and using the profile in assessing the condition of a patient.
Claims38
A method for determining whether a patient has pancreatic ductal adenocarcinoma (PDAC) comprising:
- a) measuring from a pancreatic sample from the patient the level of expression of at least two of the following diff pair miRNAs:
- miR-135b, miR148a, miR-130b, miR-196a, miR-24, miR-375, miR-96, miR-155, miR-21, miR-24, miR-201, miR-217, and miR-223, wherein at least one of the miRNAs is a biomarker miRNA and one is a comparative miRNA;
- b) determining at least one biomarker diff pair value based on the level of expression of the biomarker miRNA compared to the level of expression of the comparative miRNA;
- and,
- c) evaluating whether the pancreatic sample comprises PDAC cells based on the biomarker diff pair value(s).
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A method for characterizing pancreatic cells in a patient comprising determining the level of miR-135b expression in a biological sample comprising pancreas cells.
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A method for identifying pancreatic cancer cells in a patient comprising:
- a) obtaining a pancreatic sample from the patient,
- b) from the pancreatic sample, measuring expression levels of at least miR-135b and at least one of the following miRNAs:
- miR-24, miR-96, miR-130b, miR-148a, miR-196a, or miR-375;
- c) calculating a differential value between the level of miR-135b expression and a level of expression of a reference miRNA in the sample to provide at least one differential pair analysis factor;
- and
- d) evaluating one or more differential pair analysis factors using a scoring algorithm to generate a risk score for the presence of pancreatic cancer cells in the pancreatic sample, wherein the patient is identified as having or as not having pancreatic cancer cells based on the score.
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Dependencies are inferred from the claim text.