Nucleic acid analysis device, nucleic acid analysis apparatus, and nucleic acid analysis method

A nucleic acid analysis method comprising:

• a step of performing a nucleic acid synthesis reaction by bringing a nucleic acid synthetase and bases with a fluorescent dye into contact with a nucleic acid sample;
• and
• a step of detecting fluorescence generated by exciting a label by local illumination, wherein
• the method includes:
• a step of immobilizing a single molecule on a surface of a support base at a first point of a nucleic acid sample;
• and
• a step of immobilizing the molecule on the surface of the support base at a second point of the nucleic acid sample.

A nucleic acid analysis apparatus which determines a base sequence of a nucleic acid sample and comprises:

• a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions;
• a section which supplies at least a nucleic acid sample to the nucleic acid analysis device;
• a section which irradiates the nucleic acid analysis device with light;
• and
• a detection section which detects fluorescence incorporated by a nucleic acid extension reaction, wherein
• the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.