Method of amplifying dna from rna in sample and use thereof
AssigneeSamsung Electronics Co., Ltd.
InventorsKo-bong CHOI, Joo-won RHEE, Sea-hee KIM
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Abstract
Provided are methods of efficiently amplifying DNA from RNA in sample, methods of efficiently estimating an amount of RNA in a sample, and compositions for efficiently amplifying DNA from RNA in a sample.
Claims17
A method of amplifying DNA from RNA in a sample, the method comprising:
- incubating a sample having one or more RNA in the presence of RNA ligase, which ligates the 5′ terminal and 3′ terminal of the RNA to form circular RNA;
- combining the circular RNA with a primer hybridizable to a region of the circular RNA or a sequence complementary to the circular RNA, RNA-dependent DNA polymerase, and DNA-dependent DNA polymerase to form an aqueous component;
- introducing the aqueous component into a microcompartment of a water-in-oil emulsion;
- and
- amplifying DNA from the circular RNA.
A composition for amplifying DNA from RNA in a sample comprising a water-in-oil emulsion comprising microcompartments, wherein one or more microcompartments comprises an aqueous solution comprising one or more circular RNA, a primer that is hybridizable to a region of the circular RNA or a region complementary to the circular RNA, RNA-dependent DNA polymerase, and DNA-dependent DNA polymerase.
Dependencies are inferred from the claim text.