Method for simultaneously detecting an antigen of, and an antibody against, an infectious microorganism
AssigneeBio-Rad Innovations
InventorsFrançois RIEUNIER, Muriel Feyssaguet, Stéphanie Henriot, Nadine Lambert
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Abstract
The invention relates to a method for detecting, in vitro, an infection with a microorganism, such as the hepatitis C virus, in a biological sample, by simultaneously detecting an antigen of this microorganism and the antibodies against this same antigen, and also to the reagents and kits implementing this method.
Claims13
1-55. (canceled)
A kit which is of use for detecting an infection with human immunodeficiency virus (HIV) in a biological sample, comprising:
- a capture or detection antigen which comprises an antigenic fragment of a protein of HIV, said fragment comprising at least one destroyed epitope, while at the same time conserving the ability to bind the antibodies directed against HIV possibly present in the biological sample;
- a capture or detection antibody directed against said epitope, which is intact, of said protein of HIV.
The kit as claimed in claim 45 further comprising, besides the capture antigen, means of detecting the anti-HIV antibodies present in the biological sample, the detecting means forming complexes with the capture antigen.
The kit as claimed in claim 49, wherein the detecting means is a labeled anti-immunoglobulin or anti-isotype antibody.
The kit as claimed in claim 52, in which the capture antigen comprises an antigenic fragment of the gag protein of HIV, in which at least one epitope site has been destroyed.
Dependencies are inferred from the claim text.