Extracellular Matrix-Derived Gels and Related Methods
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsStephen F. Badylak, Donald Freytes
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Abstract
Provided are methods for preparing gelled, solubilized extracellular matrix (ECM) compositions useful as cell growth scaffolds. Also provided are compositions prepared according to the methods as well as uses for the compositions. In one embodiment a device, such as a prosthesis, is provided which comprises an inorganic matrix into which the gelled, solubilized ECM is dispersed to facilitate in-growth of cells into the ECM and thus adaptation and/or attachment of the device to a patient.
Claims28
1-96. (canceled)
A method of preparing an extracellular matrix-derived gel comprising:
- (a) solubilizing decellularized extracellular matrix (ECM) derived from cardiac tissue by digestion with an acid protease in an acidic solution to produce digested cardiac ECM;
- and (b) raising the pH of the digested cardiac ECM to a pH between 7.2 and 7.8 to produce a neutralized digest solution, and (c) gelling the neutralized digest solution at a temperature greater than 25° C.
A composition comprising extracellular matrix (ECM) derived from cardiac tissue that is a liquid at 4° C. and is a gel at a temperature greater than 25° C. and an acid protease, the composition having a pH of from 7.2 to 7.8.
A method of producing tissue in a patient, comprising implanting into a patient a composition prepared according to the method of claim 1.
A kit comprising either:
- a) a vessel containing composition comprising extracellular matrix (ECM) derived from cardiac tissue that is a liquid at 4° C. and is a gel at a temperature greater than 25° C. and an acid protease, the composition having a pH of from 7.2 to 7.8;
- or
- b) a plurality of vessels in which a first vessel of the plurality of vessels contains digested cardiac ECM prepared by solubilizing decellularized ECM derived from cardiac tissue by digestion with an acid protease in an acidic solution and a second vessel of the plurality of vessels contains a base or isotonic buffer for neutralizing the digested cardiac ECM.
Dependencies are inferred from the claim text.