Methods of inducing cell differentiation with placental extracts
AssigneeMedistem, Inc.
InventorThomas E. Ichim
Life of this patent
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Abstract
Disclosed are methods of preparing and using placentally-derived stem cells and compositions useful for the treatment of cancer. Said stem cells and compositions function through inducing a “guided differentiation” program in cancer cells, thereby reducing malignancy. Further extension of the invention pertains to augmenting ability of administered cells to induce differentiation through the co-administration of known differentiation inducing agents. Within the context of this disclosure, methods for inducing host responses to cancer are also described.
Claims22
A method of producing a cell free extract capable of inducing differentiation in cancer cells, the process comprising:
- a. extracting human placenta;
- b. homogenizing said placenta at a neutral pH, resulting in a homogenate;
- c. centrifuging said homogenate in order to free the supernatant of cellular debris;
- and
- d. purifying differentiation associated molecular weight fractions, wherein the differentiation-inducing fraction purified is of molecular weight of 10-10,000 Daltons.
A cell free extract capable of inducing differentiation in cancer cells, the cell free extract produced by the process comprising:
- a. extracting human placenta;
- b. homogenizing said placenta at a neutral pH, resulting in a homogenate;
- c. centrifuging said homogenate in order to free the supernatant of cellular debris;
- and
- d. purifying differentiation associated molecular weight fractions.
A differentiation inducing composition, suitable for administration to a patient in need thereof, derived from placental tissue, the composition produced by the process comprising:
- a. extracting human placenta;
- b. homogenizing said placenta at a neutral pH, resulting in a homogenate;
- c. centrifuging said homogenate in order to free the supernatant of cellular debris;
- d. purifying the cell free-debris through adsorption on a C-18 column;
- and
- e. filter sterilizing said composition.
A method of inhibiting progression of a neoplasm, comprising:
- providing a placental cell free extract made by the process comprising:
- extracting human placenta;
- homogenizing said placenta at a neutral pH, resulting in a homogenate;
- centrifuging said homogenate in order to free the supernatant of cellular debris;
- and
- purifying the placental cell free extract comprising a cell differentiation associated molecular weight fraction comprising a molecular weight of about 10-10,000 Daltons;
- and
- contacting said placental cell free extract with a neoplastic cell selected from the group consisting of a leukemia cell, a prostate cancer cell, and a lung cancer cell.
A method of inhibiting progression of a neoplasm, comprising:
- providing a differentiation inducing composition made by the process of:
- extracting human placenta;
- homogenizing said placenta at a neutral pH, resulting in a homogenate;
- centrifuging said homogenate in order to free the supernatant of cellular debris;
- purifying the cell free-debris through adsorption on a C-18 column;
- and
- filter sterilizing said composition to obtain a differentiation inducing composition comprising a cell differentiation associated molecular weight fraction comprising a molecular weight of about 10-10,000 Daltons;
- and
- contacting said differentiation inducing composition with a neoplastic cell selected from the group consisting of a leukemia cell, a prostate cancer cell, and a lung cancer cell.
Dependencies are inferred from the claim text.