Compositions and methods for exosome targeted expression

A cell line that produces exosomes containing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest, wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.

A method for delivering a protein of interest to a target cell in a mammal, comprising administering to the mammal an exosome comprising a Nef-fusion protein, wherein said Nef-fusion protein comprises a Nef-derived peptide fused to said protein of interest and wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.

A method for inducing an immune response in a mammal, comprising administering to a mammal an exosome comprising a Nef-fusion protein comprising a Nef-derived peptide fused to an immunogenic protein of interest, wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.

A pharmaceutical composition, comprising:

• an exosome comprising a Nef-fusion protein containing a Nef-derived peptide fused to a protein of interest;
• and
• a pharmaceutically acceptable carrier.

A Nef-fusion protein, produced by

• culturing cells that produce exosomes containing said Nef-fusion protein;
• isolating exosomes from said cells;
• and purifying said Nef-fusion protein from isolated exosomes by chromotography, wherein said Nef-fusion protein comprises a Nef-derived peptide fused to a protein of interest and wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.