Secretion System and Methods for its Use
AssigneeUniversity of Washington through its Center for Commercialization
InventorsJoseph Mougous, Alistair Brian Russell, Michele LeRoux
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Abstract
The present invention provides reagents and methods for inhibiting bacterial infection and abnormal cell growth, as well as for selection cloning of nucleic acid inserts.
Claims30
A substantially purified type VI secretion exported (Tse) protein, selected from the group consisting of Tse1, Tse2, and Tse3.
3-5. (canceled)
A host cell comprising,
- (a) a plurality of genes encoding proteins capable of forming a type 6 secretion system (T6SS);
- and
- (b) a recombinant gene encoding a therapeutic polypeptide that can be secreted by the recombinant T6SS in the recombinant cell, wherein the recombinant gene is operatively linked to a regulatory sequence.
A recombinant gene encoding a fusion polypeptide of
- (a) a therapeutic polypeptide;
- and
- (b) one or both of a VgrG polypeptide and a Hcp polypeptide.
A recombinant fusion protein comprising
- (a) a therapeutic polypeptide selected from the group consisting of bactericidal proteins group HA phospholipase A2, bactericidal/permeability-increasing protein, human peptidoglycan recognition proteins 3 and 4 (PGLYRP3 and PGLYRP4), Tse1, Tse2, and Tse3;
- and
- (b) one or both of a VgrG polypeptide and a Hcp polypeptide.
(canceled)
(canceled)
A method for improved biomolecule extraction from bacterial cells, comprising contacting the bacterial cells with an amount effective of Tse1 to lyse the bacterial cells during the extraction process.
(canceled)
A recombinant vector, comprising a first gene coding for type VI secretion exported protein 1 (Tse1) or, type VI secretion exported protein 1 (Tse3) wherein the first gene is operatively linked to a heterologous regulatory sequence.
21-23. (canceled)
25-26. (canceled)
28-31. (canceled)
A recombinant vector, comprising a nucleic acid encoding Tsi1 or Tsi3, wherein the nucleic acid is operatively linked to a regulatory sequence.
A host cell comprising in its genome, a first recombinant gene coding for Tse1 or Tse3 operatively linked to a regulatory sequence.
36-38. (canceled)
A substantially purified protein, selected from the group consisting of Tsi1, Tsi2, and Tsi3.
A method for improved biomolecule extraction from bacterial cells, comprising contacting the bacterial cells with an amount effective of Tse1 to lyse the bacterial cells during the extraction process.
Dependencies are inferred from the claim text.