Compositions and methods for preparing nanoformulations and systems for nano-delivery using focused acoustics

A method of preparing a formulation, comprising:

• providing a mixture in a vessel, the mixture comprising a first composition and a second composition;
• transmitting focused acoustic energy having a frequency of between about 100 kilohertz and about 100 megahertz and a focal zone having a size of less than about 2 centimeters through a wall of the vessel such that the mixture is disposed at least partially in the focal zone;
• selecting one or more parameters of the focused acoustic energy to achieve a desired particle size of an emulsion or liposome-containing formulation created by exposing the mixture to the focused acoustic energy, different parameters of the focused acoustic energy providing different particle sizes in the emulsion or liposome-containing formulation after exposure to the focused acoustic energy;
• and
• forming a plurality of particles in the mixture to create an emulsion or liposome-containing formulation by, at least in part, exposure of the mixture to the focal zone of the focused acoustic energy having the selected one or more parameters, a plurality of liquid particles in the emulsion or plurality of liposome particles in the liposome-containing formulation having an average size of between approximately 10 nm and approximately 400 nm after exposure of the mixture to the focal zone.