Generation of engineered molecular weight standards
AssigneeBio-Rad Laboratories, Inc.
InventorsLEI LI, DENNIS YEE
Life of this patent
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Abstract
The invention provides methods of generating physical analog polypeptides, and polypeptides generated employing the methods of the invention, that have the same amino acid composition of a reference protein, but have an unrelated primary sequence. The invention further provides compositions and methods employing such proteins.
Claims30
An engineered protein comprising a physical analog of a reference protein amino acid sequence, wherein the analog:
- (i) has the same amino acid composition as the reference protein sequence;
- and (ii) has a primary sequence that has less than 50% identity to the reference protein sequence.
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An engineered polypeptide that comprises an amino acid sequence having at least 80% identity to an amino acid sequence set forth in SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8;
- wherein the protein characterized by the amino acid sequence retains the same charge and molecular weight as a reference protein set forth in SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, or SEQ ID NO:7.
A molecular weight marker composition comprising an aqueous solution and at least one engineered polypeptide comprising a physical analog of a reference protein amino acid sequence, wherein the analog:
- (i) has the same amino acid composition as the reference protein sequence;
- and (ii) has a primary sequence that has less than 50% identity to the reference protein sequence.
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A molecular weight marker composition that comprises an engineered polypeptide that comprises an amino acid sequence having at least 80% identity to an amino acid sequence set forth in SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8;
- wherein the protein characterized by the amino acid sequence retains the same charge and molecular weight as a reference protein set forth in SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, or SEQ ID NO:7.
A method of generating a physical analog polypeptide, the method comprising providing a reference protein sequence;
- reversing and/or scrambling a subsequence of the reference protein to obtain a physical analog sequence wherein the analog sequence:
- (i) has the same amino acid composition as the reference protein sequence;
- and (ii) has a primary sequence that has less than 50% identity to the reference protein sequence;
- and
- synthesizing a protein comprising the physical analog polypeptide.
Dependencies are inferred from the claim text.