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US 2013/0153209 A1Published application
Shewanella enrichment from oil reservoir fluids
OwnerEdwin Hendrickson, Abigail Luckringinventors
Life of this patent
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Abstract
Methods for enriching microbial populations that are indigenous to oil reservoir fluids to obtain Shewanella-enriched populations and isolated Shewanella strains useful for enhancing oil recovery from an oil reservoir are presented. In addition, the enriched populations may contain bacteria belonging to the Arcobacter genus.
Claims11
A method for enriching a microbial population comprising:
- a) providing an environmental sample from an oil reservoir fluid comprising an indigenous microbial population;
- and
- b) growing said sample using medium comprising salt, at least one carbon source, and an electron acceptor selected from organic electron acceptors and metal ion electron acceptors to obtain an enriched microbial population;
- wherein the proportion of Shewanella cells in the enriched microbial population of (b) is greater than the proportion of Shewanella cells in the environmental sample of (a).
1 more element
A method for isolating a strain of the Shewanella genus comprising:
- a) providing an environmental sample from an oil reservoir fluid comprising an indigenous microbial population;
- b) growing said sample using medium comprising salt, at least one carbon source, and an electron acceptor selected from organic electron acceptors and metal ion electron acceptors to obtain an enriched microbial population;
- c) growing the enriched microbial population of (b) on medium having salt concentration that is between about 10 ppt and 40 ppt to obtain isolated colonies;
- and
- d) screening the isolated colonies of (c) by rDNA sequence analysis against known microbial rDNA sequences;
- wherein at least one Shewanella strain is identified.
3 more elements
Dependencies are inferred from the claim text.