Transgenic plants expressing a viral antifungal protein
AssigneeDonald Danforth Plant Science Center
InventorsThomas James Smith, Dilip Shah
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Abstract
Transgenic plants expressing the KP4 antifungal protein are provided which exhibit high levels of antifungal resistance. Such transgenic plants contain a recombinant DNA construct comprising a heterologous signal peptide sequence that is oper ably linked to a non-native nucleic acid sequence encoding a mature KP4 antifungal protein.
Claims36
A transgenic plant comprising a recombinant nucleic acid construct, said recombinant nucleic acid construct comprising a promoter that is operably linked to a nucleic acid encoding a heterologous signal peptide that is operably linked to a non-native nucleic acid encoding a KP4 antifungal protein that is operably linked to a polyadenylation sequence, wherein said transgenic plant expresses said KP4 protein in the apoplast and provides for at least 50% inhibition of a plant pathogenic fungal infection relative to a control plant that lacks said recombinant nucleic acid construct.
A transgenic maize plant comprising a chromosome containing an insertion of a KP4 protein expression cassette of plasmid pZP212-KP4, wherein said insertion provides for at least 50% inhibition of a plant pathogenic fungal infection relative to a control maize plant that lacks said recombinant nucleic acid construct.
A transgenic soybean plant comprising a chromosome containing an insertion of an FMV promoter to a chimeric KP4 protein encoding region of SEQ ID NO:7, wherein said insertion provides for at least 50% inhibition of a plant pathogenic fungal infection relative to a control soybean plant that lacks said recombinant nucleic acid construct.
Dependencies are inferred from the claim text.