Axmi115 variant insecticidal gene and methods for its use
OwnerVolker Heinrichs, Duane Lehtineninventors
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OwnerVolker Heinrichs, Duane Lehtineninventors
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Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof.
A recombinant nucleic acid molecule comprising a nucleotide sequence encoding a fusion polypeptide having pesticidal activity, wherein the fusion polypeptide comprises a C-terminal portion of SEQ ID NO:43.
A recombinant polypeptide having pesticidal activity, wherein the polypeptide comprises a C-terminal portion of SEQ ID NO:43.
A plant having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule comprising a nucleotide sequence encoding a fusion polypeptide having pesticidal activity, wherein the fusion polypeptide comprises a C-terminal portion of SEQ ID NO:43.
A method for protecting a plant from a pest, comprising expressing in a plant or cell thereof a nucleic acid molecule comprising a nucleotide sequence encoding a fusion polypeptide having pesticidal activity, wherein the fusion polypeptide comprises a C-terminal portion of SEQ ID NO:43.
A method for increasing yield in a plant comprising growing in a field a plant of or a seed thereof having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule comprising a nucleotide sequence encoding a fusion polypeptide having pesticidal activity, wherein the fusion polypeptide comprises a C-terminal portion of SEQ ID NO:43.
Dependencies are inferred from the claim text.