Method for purifying protein using amino acid
AssigneeKyowa Hakko Kirin Co., Ltd.
InventorTakashi Ishihara
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Abstract
In large-scale purification of proteins such as antibodies, an economic high-purity purification method is required. The present invention relates to a method for purifying a protein, including one or more chromatographic processes, in which an amino acid; or a dipeptide, an oligopeptide, or a polyamino acid thereof is included in a buffer solution used in at least one chromatographic processes (equilibration buffer, wash buffer, and elution buffer), thereby purifying a high-purity protein with a very small quantity of the impurity (e.g., polymers or host cell proteins).
Claims16
A method for purifying a protein, comprising one or more chromatographic processes, wherein an amino acid is included as an ingredient of a buffer solution or a part thereof used in at least one chromatographic process.
Dependencies are inferred from the claim text.