Process for the purification of a growth factor protein
AssigneeOctapharma AG
InventorsGustav Gilljam, Stefan Winge, Maya Tiemeyer
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Abstract
A process of purifying a Growth Factor Protein in a purification sequence employing chromatography characterized in that at least one chromatography is performed using a multimodal resin the Growth Factor Protein binds to the multimodal resin at a pH between 4 to 6.2, and the Growth Factor Protein is eluting at a pH>6.3, and the elution of Growth Factor Protein is improved by addition of arginine and/or NaCl to the eluting buffer. The multimodal resin step is followed by a yeast derived affinity ligand resin step, which results of a purity of the product>90%.
Claims25
A process of purifying a Growth Factor Protein selected from the group consisting of Colony Stimulating Factor (CSF), interleukin 3 (IL-3), Hepatocyte growth factor, Epidermal growth factor and fibroblast growth factor (acid) in a purification sequence employing chromatography comprising
- binding the Growth Factor Protein to a multimodal resin at a pH between 4 to 6.2, and
- eluting the Growth Factor Protein at a pH>6.3.
Dependencies are inferred from the claim text.