Method of detecting tau protein and tau fragments in serum
AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorsJohn F. DeBernardis, Raymond P. Zinkowski
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Abstract
The present invention provides quantitative methods for the detection of tau protein and/or tau fragments. More specifically, the present invention provides quantitative methods for diagnosing a tauopathy or ruling out a tauopathy as the cause of disease, particularly the diagnosis and ruling of Alzheimer's disease. The present invention further provides a method for diagnosing a tauopathy by computing the ratio of two detected tau proteins or tau fragments.
Claims26
A method for determining the amount or presence of at least one tau protein or tau fragment in a biological sample comprising:
- (a) contacting the sample with a first antibody and a second antibody to form a complex wherein both the first antibody and the second antibody bind specifically to tau polypeptide;
- and
- (b) detecting the presence or amount of the complex, wherein the presence or amount of the complex is indicative of the presence or amount of tau polypeptides and wherein
- (i) the first antibody is specific to the tau epitope 180-210 and the second antibody is specific to the tau epitope 150-170, or
- (ii) the first antibody is specific to the tau epitope 192-204 and the second antibody is specific to the tau epitope 159-163, or
- (iii) the first antibody is specific to the tau epitope 200-250 and the second antibody is specific to the tau epitope 150-170, or
- (iv) the first antibody is specific to the tau epitope 210-230 and the second antibody is specific to the tau epitope 159-163, or
- (v) the first antibody is specific to the tau epitope 102-150 and the second antibody is specific to the tau epitope 150-170, or
- (vi) the first antibody is specific to the tau epitope 102-150 and the second antibody is specific to the tau epitope 159-163.
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A method of detecting a tauopathy comprising
- (a) determining the amount of a tau protein or tau fragment in a sample;
- and
- (b) determining the ratio of at least two tau fragments or tau proteins.
A method of detecting tauopathy by determining the amount of a tau protein or a tau fragment in a biological sample comprising:
- (a) contacting the sample with a first antibody and a second antibody to form a complex wherein both the first antibody and the second antibody bind specifically to tau polypeptide;
- and
- (b) detecting the presence or amount of the complex wherein the presence or amount of the complex is indicative of the presence or amount of tau polypeptide;
- and
- (c) determining the ratio of at least two detected tau fragments or tau proteins.
A method for determining the amount or presence of at least one tau protein or tau fragment in a biological sample comprising:
- (a) contacting the sample with a first antibody and a second antibody to form a complex wherein both the first antibody and the second antibody bind specifically to tau polypeptide;
- and
- (b) detecting the presence or amount of the complex, wherein the presence or amount of the complex is indicative of the presence or amount of tau polypeptide;
- and
- (c) determining the ratio of at least two detected tau fragments or tau proteins to rule out Alzheimer's disease in a patient.
Dependencies are inferred from the claim text.