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US 2013/0095187 A1Published application
Composition for nucleic acid delivery using metal nanoparticles and preparing method thereof
AssigneePostech Academy-Industry Foundation
InventorsSei Kwang HAHN, Min-Young Lee, Kitae Park, Ku Su Kim, Hwiwon Lee
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Abstract
The present invention relates to a composition for nucleic acid delivery and a method for preparing the same, more particularly to a composition for nucleic acid delivery having excellent stability in the body environment and excellent intracellular delivery efficiency of nucleic acid, and enabling target directed delivery of nucleic acid, and a method for preparing the same.
Claims22
A composition for nucleic acid delivery comprising
- positively charged metal nanoparticles;
- and
- a nucleic acid layer, a cationic polymer layer and an anionic polymer layer formed by coating of nucleic acid, cationic polymer and anionic polymer on the surface of the metal nanoparticles by electrostatic layer by layer self assembly.
A method for preparing a composition for nucleic acid delivery, comprising:
- reacting metal nanoparticles with positive charge introducing material to introduce positive charge on the surface of the metal nanoparticles;
- coating nucleic acid on the surface of the positive charge introduced metal nanoparticle surface to form a metal nanoparticle/nucleic acid composite;
- coating cationic polymer on the metal nanoparticle/nucleic acid composite to form a metal nanoparticle/nucleic acid/cationic polymer composite;
- and
- coating anionic polymer on the metal nanoparticle/nucleic acid/cationic polymer composite to form a metal nanoparticle/nucleic acid/cationic polymer/anionic polymer composite,
- wherein the nucleic acid, cationic polymer, and anionic polymer is coated by electrostatic layer by layer self assembly.
3 more elements
Dependencies are inferred from the claim text.