Recombinant production of authentic human proteins using human cell expression systems
AssigneeHumanzyme Limited
InventorsRidong Chen, Soon Seog Jeong, Hui Feng
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Abstract
The present invention relates to novel expression cassettes and vectors for efficiently producing authentic recombinant human proteins from stable cultures of novel human cell lines, the authentic recombinant proteins produced therefrom, and antibodies raised against those authentic recombinant proteins.
Claims25
-65. (canceled)
An isolated recombinant authentic human cytokine, wherein the cytokine comprises LEFTY1 or LEFTY2.
An isolated recombinant authentic human cytokine, wherein the cytokine is produced by a human cell comprising an expression vector comprising a cassette comprising the following operably linked expression elements:
- (A) a human cytomegalovirus immediate early enhancer element;
- (B) a functional human beta-actin promoter;
- (C) a human globin gene intron;
- and
- (D) a human signal peptide consisting of a immunoglobulin superfamily 8 signal peptide, or an alpha-fibrinogen signal peptide,
- wherein the cytokine is encoded by and expressed from the vector, and
- wherein the cytokine comprises LEFTY1 or LEFTY2.
A composition comprising an isolated recombinant authentic human cytokine produced by a human cell comprising an expression vector comprising a cassette comprising the following operably linked expression elements:
- (A) a human cytomegalovirus immediate early enhancer element;
- (B) a functional human beta-actin promoter;
- (C) a human globin gene intron;
- and
- (D) a human signal peptide consisting of a immunoglobulin superfamily 8 signal peptide, or an alpha-fibrinogen signal peptide,
- wherein the cytokine is encoded by and expressed from the vector, and
- wherein the cytokine comprises LEFTY1 or LEFTY2.
Dependencies are inferred from the claim text.