Nitrogen responsiveness in plants through the expression of pathways for the formation and catabolism of novel n-rich compounds
AssigneePioneer Hi-Bred International, Inc.
InventorsRAJEEV GUPTA, KANWARPAL SINGH DHUGGA
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Abstract
The invention provides for the regulation of opine synthesis and catabolism providing improved nitrogen responsiveness, utilizing opine synthase and oxidase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering nitrogen utilization and/or uptake in plants. The invention further provides recombinant expression cassettes, host cells and transgenic plants.
Claims34
The use of an isolated polynucleotide selected from the group consisting of:
- a. a polynucleotide having at least 90% sequence identity, as determined by the GAP algorithm under default parameters, to the full length sequence of a polynucleotide selected from the group consisting of SEQ ID NOS:1, 3, 5, 7, 9 and 11, wherein the polynucleotide encodes a polypeptide that functions as a modifier of nitrogen utilization efficiency;
- b. a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NO:
- 2, 4, 6, 8, 10 and 12;
- c. a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1, 3, 5, 7, 9 and 11;
- and
- d. A polynucleotide which is complementary to the polynucleotide of (a), (b) or (c), to modify opine expression in plants.
A method of increasing the opine metabolism polypeptides activity in a plant cell, comprising:
- a. providing a nucleotide sequence comprising at least 15 consecutive nucleotides of the complement of SEQ ID NO:
- 1, 3, 5, 7, 9 or 11;
- b. providing a plant cell comprising an mRNA having the sequence set forth in SEQ ID NO:
- 1, 3, 5, 7, 9 or 11;
- and
- c. introducing the nucleotide sequence of step (a) into the plant cell of step (b), wherein the nucleotide sequence increase the expression of the mRNA in the plant cell.
The use of the expression cassette of claim 33, wherein said seed-specific promoter is an embryo specific promoter.
The use of the expression cassette of claim 33, wherein said seed-specific promoter is an endosperm-specific promoter.
Dependencies are inferred from the claim text.