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AssigneeU.S. Smokeless Tobacco Company LLC
InventorsDongmei Xu, Mark T. Nielsen, Yanxin Shen
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The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nomicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.
A tobacco hybrid comprising plants having a mutation in a gene encoding a cytochrome p450 CYP82E4 polypeptide having nicotine demethylase activity, wherein the mutation comprises a stop codon at the residue that aligns with the tryptophan at position 329 in SEQ ID NO:2, wherein the plants exhibit a nonconverter phenotype of less than 5% nicotine demethylation.
A tobacco variety comprising plants having a mutation in a gene encoding a cytochrome p450 CYP82E4 polypeptide having nicotine demethylase activity, wherein the mutation comprises a stop codon at the residue that aligns with the tryptophan at position 329 in SEQ ID NO:2, wherein the plants exhibit a nonconverter phenotype of less than 5% nicotine demethylation.
A method of making a tobacco plant, comprising the steps of:
A method for producing a tobacco plant comprising the steps of:
Dependencies are inferred from the claim text.