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US 2013/0028981 A1Published application
Methods for tissue decellularization
AssigneeDecell Technologies Inc.
InventorPaul F. Gratzer
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AssigneeDecell Technologies Inc.
InventorPaul F. Gratzer
Estimating the term…
A method for producing sterile, decellurized bioprosthetic tissue comprising: (a) contacting a human tissue with a hypotonic solution to produce a lysed tissue, (b) contacting the lysed tissue with a first surfactant to produce a surfactant-treated tissue, (c) contacting the surfactant-treated tissue with a nuclease enzyme solution to produce an enzyme-treated tissue, (d) contacting the enzyme-treated tissue with a cleaning solution comprising a second surfactant, a chaotropic agent or a mixture thereof to produce a decellurized tissue and (e) contacting the decellurized tissue with a bioburden reducing agent to produce the final bioprosthetic tissue. Kits to be used in conjunction with said method, as well as, the bioprosthetic tissue produced via said method are also provided.
A method for producing a human bioprosthetic tissue comprising:
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A bioprosthetic tissue, wherein the tissue is substantially free of nucleic acids, wherein the tissue is substantially free of major histocompatibility molecules (MHC), wherein the tissue is substantially free of staphylococcus bacteria, wherein the tissue is substantially free of streptococcus bacteria, wherein the tissue is substantially free of enterococcus bacteria, wherein the tissue is substantially free of bacillus bacteria, and wherein the collagen structure of the tissue is not substantially altered compared to a fresh control tissue.
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A kit, comprising a hyptotonic solution, a first surfactant solution, a nuclease enzyme solution, a cleaning solution, a bioburden reducing agent solution, and instructions for contacting a human tissue with each of the solutions.
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Dependencies are inferred from the claim text.