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US 2012/0282654 A1Published application
Antibody purification
AssigneeSchering Corporation
InventorsYan Yao, Igor Quinones-Garcia, Patricia Rowicki, John Richard Gavin, Collette Marie Cutler, Steven J. Blaisdell
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Abstract
The present invention provides, in part, a multi-step process for purifying antibodies including chromatographic purification and filter purification.
Claims25
A method for purifying an antibody or antigen-binding fragment thereof in an aqueous composition that specifically binds IGF1R which comprises a CDR-L1, CDR-L2 and CDR-L3 found in a light chain immunoglobulin variable region which comprises the amino acid sequence set forth in SEQ ID NO:
- 1;
- and a CDR-H1, CDR-H2 and CDR-H3 found in a heavy chain immunoglobulin variable region which comprises the amino acid sequence set forth in SEQ ID NO:
- 2;
- which method comprises:
- (a) purifying the antibody by protein A chromatography;
- (b) inactivating virus particles in the composition;
- (c) purifying the antibody by cation-exchange chromatography;
- (d) purifying the antibody anion-exchange chromatography;
- (e) filtering virus particles from the composition;
- (f) ultrafiltering the composition;
- (g) diafiltering the composition;
- and
- (h) fine filtering the composition.
10 more elements
Dependencies are inferred from the claim text.