Production of recombinant factor ix in a human hepatocyte cell line
AssigneeUniversite Claude Bernard Lyon I
InventorsNathalie Enjolras, Claude Negrier, Yesim Dargaud
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Abstract
The invention relates to a recombinant human factor IX (FIX) characterized in that said factor is obtained by a preparation method comprising, or even consisting of, the steps which consist in causing the genetic material encoding the FIX to be expressed in vitro in a human hepatocyte cell line Huh7, recovering the cellular supernatant in which the FIX was secreted and, optionally, purifying the synthesized FIX.
Claims12
A recombinant human factor IX (FIX) characterized in that it is obtained by means of a preparation method comprising, or even consisting of, the steps which consist in:
- causing the genetic material encoding the FIX to be expressed in vitro in a human hepatocyte cell line Huh7,
- recovering the cell supernatant in which the FIX was secreted, and
- optionally, purifying the synthesized FIX.
A method for preparing a human hepatocyte cell line producing biologically active recombinant human FIX, characterized in that it comprises, or even consists of, the following steps:
- causing the genetic material encoding the FIX to be expressed in vitro in said human hepatocyte cell line Huh7,
- recovering the cell supernatant in which the FIX was secreted, and
- optionally, purifying the synthesized FIX.
A recombinant factor IX produced by the Huh7-CD4 cell line deposited and registered under number I-4234, with the CNCM [National Collection of Microorganism Cultures].
A cell line Huh7-CD4 deposited and registered under number I-4234, with the CNCM.
Dependencies are inferred from the claim text.