Compositions and methods for detecting and identifying salmonella enterica strains
AssigneeLife Technologies Corporation
InventorsCraig Cummings, Elena Bolchakova, Manohar Furtado
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Abstract
The present specification describes several novel SNPs of Salmonella enterica subsp. enterica. SNP profiles comprising allelic compositions at each SNP position are described which may be used to identify and differentiate different strains and serovars of Salmonella enterica subsp. enterica. The specification also describes several compositions, methods and kits useful for identifying and differentially distinguishing strains and serovars of Salmonella enterica subsp. enterica.
Claims18
A method of identifying a strain or serovar of Salmonella enterica in a sample comprising:
- determining an allele corresponding to a single nucleotide polymorphisms (SNP) for at least ten SNPs from nucleic acids isolated from the sample;
- and
- determining the allelic composition for the at least ten SNPs selected from a panel of SNPs, wherein the presence of certain alleles identifies the strain or serovar of Salmonella enterica.
An isolated nucleotide sequence comprising at least 15 nucleotides of SEQ ID NO:
- 1-SEQ ID NO:
- 52, comprising at least nucleotides located at positions 100-103 of SEQ ID NO:
- 1-SEQ ID NO:
- 52.
An isolated nucleotide sequence comprising SEQ ID NO:
- 54-SEQ ID NO:
- 157.
An isolated nucleotide sequence comprising SEQ ID NO:
- 158-SEQ ID NO:
- 261.
Dependencies are inferred from the claim text.