Methods of generating neural stem cells
AssigneeThe J. David Gladstone Institutes
InventorsYadong Huang, Karen Ring
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods of generating neural stem cells from differentiated somatic cells. The present disclosure also provides induced neural stem cells generated using a subject method, as well as differentiated cells generated from a subject induced neural stem cell. A subject neural stem cell, as well as differentiated cells derived from a subject neural stem cell, is useful in various applications, which are also provided in the present disclosure.
Claims33
A method of generating a multipotent induced neural stem cell, the method comprising:
- a) introducing an exogenous Sox2 polypeptide into a somatic cell, to generate an exogenous Sox2-modified somatic cell that comprises exogenous Sox polypeptide;
- and
- b) culturing the exogenous Sox2-modified somatic cell on a protein-coated solid substrate, wherein said solid substrate comprises a material other than a plastic, wherein said introducing and said culturing results in generation of a multipotent induced neural stem cell (iNSC).
A method for performing cell transplantation in a subject in need thereof, the method comprising:
- (i) generating an induced neural stem cell from a somatic cell of a healthy donor individual, wherein the donor individual is immunocompatible with the subject;
- and (ii) transplanting one or more cells differentiated from the induced neural stem cell into the subject.
Dependencies are inferred from the claim text.