Novel bacillus thuringiensis insecticidal proteins
AssigneeBayer CropScience AG
InventorsGreta Arnaut, Annemie Boets, Stijn Vanneste, Jeroen Van Rie, Sara Van Houdt
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Abstract
The invention pertains to novel insecticidal compounds derived from Bacillus thuringiensis strains. New proteins designated Cry2Ae, Cry2Af, and Cry2Ag, and variants thereof are provided, as well as DNA sequences encoding these proteins or their variants. Further provided are recombinant hosts expressing such proteins, particularly plant cells and plants.
Claims21
1-56. (canceled)
A method for controlling Helicoverpa armigera, Anticarsia gemmatalis or Sesamia inferens comprising applying an insecticidal protein to an area or plant to be protected, wherein said area or plant is a habitat of Helicoverpa armigera, Anticarsia gemmatalis or Sesamia inferens, and wherein said insecticidal protein is
- i) an insecticidal protein comprising an amino acid sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO. 2,
- ii) an insecticidal protein comprising the amino acid sequence from the amino acid at position 1 to the amino acid at position 625 in SEQ ID No. 2,
- iii) an insecticidal protein comprising the amino acid sequence from the amino acid at position 50 to the amino acid at position 625 in SEQ ID No. 2,
- iv) an insecticidal protein comprising the amino acid sequence of SEQ ID No. 2,
- v) an insecticidal protein having 5-10 amino acids added, replaced or deleted in the sequence of SEQ ID NO:
- 2 without changing the insecticidal activity of the protein,
- vi) an insecticidal protein having less than 5 amino acids added, replaced or deleted in the sequence of SEQ ID NO:
- 2 without changing the insecticidal activity of the protein,
- vii) an insecticidal protein comprising the amino acid sequence of SEQ ID No. 8, or
- viii) an insecticidal protein encoded by the DNA of SEQ ID No 7 or 9.
An isolated DNA sequence encoding a truncated insecticidal Cry2Ae protein consisting of the amino acid sequence of the protein of SEQ ID NO:
- 2 from amino acid position 1 to an amino acid position between amino acid position 625 and amino acid position 632.
Dependencies are inferred from the claim text.