Transdermal systems, devices, and methods to optically analyze an analyte

A transdermal optical analysis test system for non-invasively determining the concentration of at least one analyte in a fluid, the system comprising:

• a transdermal test sensor, where the transdermal test sensor comprises:
• a housing at least partially enclosing an aqueous material, the aqueous material comprising at least one analyte selective reagent and at least one optically active moiety,
• the at least one analyte selective reagent comprising at least one of a receptor and one or more species that the analyte displaces from the receptor, an analyte binding moiety that undergoes a conformation change when contacted by the analyte, an analyte binding moiety that undergoes a change in electron withdrawing ability when contacted by the analyte, and an analyte binding moiety that produces at least one byproduct when contacted by the analyte,
• the at least one optically active moiety comprising at least one of a pH sensitive dye, an oxygen sensitive dye, a donor fluorescent dye and an acceptor fluorescent dye in combination, and a donor fluorescent dye and a quencher in combination;
• an optical analysis system comprising a light source and a detector;
• and
• a measurement device.

A transdermal optical analysis test sensor for non-invasively determining the concentration of at least one analyte in a fluid, the test sensor comprising:

• a housing at least partially enclosing an aqueous material, the aqueous material comprising at least one analyte selective reagent and at least one optically active moiety,
• the at least one analyte selective reagent comprising at least one of a receptor and one or more species that the analyte displaces from the receptor, an analyte binding moiety that undergoes a conformation change when contacted by the analyte, an analyte binding moiety that undergoes a change in electron withdrawing ability when contacted by the analyte, and analyte binding moiety that produces at least one byproduct when contacted by the analyte,
• the at least one optically active moiety comprising at least one of a pH sensitive dye, an oxygen sensitive dye, a donor fluorescent dye and an acceptor fluorescent dye in combination, and a donor fluorescent dye and a quencher in combination;
• and
• an optical analysis system comprising a light source and a detector.

A transdermal optical analysis test system for non-invasively determining the concentration of at least one analyte in a fluid, the system comprising:

• means for contacting porated tissue with an aqueous material of a transdermal test sensor;
• means for allowing a fluid sample to traverse the porated tissue and enter the aqueous material;
• means for applying at least one wavelength of light to the aqueous material;
• means for detecting at least one wavelength of light from the aqueous material;
• and
• means for correlating the alteration between the applied and detected light with the concentration of the at least one analyte in the sample.