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US 2012/0258460 A1Published application
Sso7-Polymerase Conjugates with Decreased Non-Specific Activity
AssigneeBio-Rad Laboratories, Inc.
InventorsMan Cheng, Yan Wang, John Sullivan
Life of this patent
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Abstract
Improved Sso7-polymerase conjugate proteins are provided
Claims20
An Sso7 polymerase conjugate protein comprising an Sso7 domain linked to a polymerase;
- wherein:
- an amino acid of the Sso7 domain corresponding to K28 of SEQ ID NO:2 is not lysine (K);
- and/or
- an amino acid of the Sso7 domain corresponding to R43 of SEQ ID NO:2 is not arginine (R),
- wherein the conjugate protein has a decreased non-specific amplification activity compared to an otherwise identical control conjugate protein in which the amino acid of the Sso7 domain corresponding to K28 of SEQ ID NO:2 is lysine (K) and the amino acid of the Sso7 domain corresponding to R43 of SEQ ID NO:2 is arginine (R).
2 more elements
Dependencies are inferred from the claim text.