Bisulfite conversion of DNA
AssigneeEpigenomics AG
InventorsIna Fuhrmann, Matthias Ballhause
Life of this patent
Estimating the term…
Abstract
The present invention relates to an improved method for the bisulfite conversion of DNA. In certain time-temperature ranges the efficacy of the bisulfite conversion is clearly improved. By combination with denaturating solvents, new reaction conditions and new purification methods the efficacy can be further increased The converted DNA can subsequently be analysed by different methods. The present invention facilitates the analysis of cytosine methylation.
Claims30
A method for bisulfite conversion of DNA, wherein the bisulfite reaction is conducted at a temperature >50° C. and with a reaction time ≧5 h.
A method for bisulfite conversion of DNA, wherein the bisulfite reaction is conducted at a temperature >55° C. and with a reaction time ≧5 h.
A method for bisulfite conversion of DNA, wherein the bisulfite reaction is conducted at a temperature of 60° C. for 5 h or at a temperature of 55° C. for 7 h.
28-40. (canceled)
Kit comprising a bisulfite reagent, a radical scavenger, microfiltration tubes, primer, polymerase and compounds necessary for the bisulfite conversion, purification and/or amplification.
Dependencies are inferred from the claim text.