Cell lysis apparatus and manufacturing method thereof

A method of manufacturing a mechanical cell lysis apparatus, the mechanical cell lysis apparatus comprising an inlet port through which fluid containing cells is supplied;

• a fluid channel defining a passageway through which the cell containing fluid supplied from the inlet port flows;
• a nano blade array including a plurality of nano blades having pointed leading ends and arranged such that the pointed leading ends of the nano blades are oriented in a direction of the fluid supplied through the inlet port to disrupt the cells passing therethrough;
• and an outlet port through which the disrupted cells and the fluid are discharged, the method comprising:
• preparing a main panel comprising a silicon substrate;
• forming an etching mask on an upper side of the silicon substrate;
• and
• forming the nano blade array and the fluid channel through crystalline wet etching, with sidewalls of the nano blade array arranged in a particular direction with respect to a particular plane of the silicon substrate, such that the nano blades have pointed leading ends.