US 2012/0238739 A1Published application

Methods for improved dna release from binding substrates and/or decreasing pcr inhibition in pathogen detection

AssigneeE.I. du Pont de Nemours and Company

InventorsT. JOSEPH DENNES, MICHAEL P. PERRY

Life of this patent

Estimating the term…

Loading patent…

Abstract

Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface.

Claims14

Dependent claims

A process for collecting nucleic acids from a particulate sample comprising:

(a) transferring an aliquot of a particulate sample containing nucleic acids into a device comprising
(i) a chamber interior comprising a fibrous nucleic acid binding surface, the chamber interior being capable of expanding in size in at least one dimension;
and
(ii) a nucleic acid binding solution;
the fibers of the fibrous nucleic acid binding surface expanding to at least partially fill the chamber interior upon wetting with the particulate sample and the nucleic acid binding solution;
(b) mixing the particulate sample with the nucleic acid binding solution;
(c) expelling the nucleic acid binding solution from the device by compression of the fibrous nucleic acid binding surface while retaining the fibrous nucleic acid binding surface in the chamber interior;
(d) powderizing the fibrous nucleic acid binding surface;
(e) contacting an elution buffer with the powderized nucleic acid binding surface;
and
(f) separating the elution buffer from the powderized nucleic acid binding surface, whereby the elution buffer contains nucleic acids from the particulate sample.

8 more elements

Dependencies are inferred from the claim text.