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US 2012/0238030 A1Published application
Methods and Systems for Multiplex Analysis of Biomolecules by Liquir Chromatography-Mass Spectrometry
AssigneeLaboratory Corporation of America Holdings
InventorsBrian Alexander Rappold, Russell Philip Grant
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Abstract
Multiplex analysis methods for rapid analysis of the presence or amount of two or more biomolecules in a sample are disclosed. Systems implementing such methods are further disclosed.
Claims29
A method for analyzing the presence or amount of two or more biomolecules in a sample, the method comprising:
- (a) providing a sample comprising a first biomolecule and a second biomolecule;
- (b1) chromatographically separating the first biomolecule from other components in the sample using a first liquid chromatography column, and (b2) chromatographically separating the second biomolecule from other components in the sample using a second liquid chromatography column;
- wherein the first liquid chromatography column and the second liquid chromatography column employ different column chemistries;
- and
- (c) analyzing the chromatographically separated first biomolecule and the chromatographically separated second biomolecule by mass spectrometry to determine the presence or amount of the first biomolecule and the second biomolecule in the sample.
2 more elements
A method for analyzing the amount of two or more amino acids in a sample, the method comprising:
- (a) providing a sample, the sample comprising a sample that contains a first amino acid and a second amino acid;
- (b) deproteinating the sample;
- (c1) chromatographically separating the first amino acid from other components in the sample using a first liquid chromatography column, and (c2) chromatographically separating the second amino acid from other components in the sample using a second liquid chromatography column, wherein the first liquid chromatography column and the second liquid chromatography column employ different column chemistries;
- and
- (d) analyzing the chromatographically separated first amino acid and the chromatographically separated second amino acid by mass spectrometry to determine the amount of the first amino acid and the second amino acid in the sample.
2 more elements
A method generating a report for diagnosing a disease or condition associated with an abnormal level of an amino acid in a subject, the method comprising:
- (a) providing a sample, the sample comprising a sample that contains a first amino acid and a second amino acid;
- (b) deproteinating the sample;
- (c1) chromatographically separating the first amino acid from other components in the sample using a first liquid chromatography column, and (c2) chromatographically separating the second amino acid from other components in the sample using a second liquid chromatography column, wherein the first liquid chromatography column and the second liquid chromatography column employ different column chemistries;
- (d) analyzing the chromatographically separated first amino acid and the chromatographically separated second amino acid by mass spectrometry to determine the amount of the first amino acid and the second amino acid in the sample;
- and
- (e) generating a report that recites the concentration of at least one of the first amino acid or the second amino acid in the sample.
3 more elements
A system for analyzing of two or more biomolecules in a sample, the system comprising:
- (a) a sample, the sample comprising a first biomolecule and a second biomolecule;
- (b) a station for chromatographically separating the first biomolecule from other components in the sample using a first liquid chromatography column, and for separating the second biomolecule from other components in the sample using a second liquid chromatography column, wherein the first liquid chromatography column and the second liquid chromatography column employ different column chemistries;
- and
- (c) a station for analyzing the chromatographically separated first biomolecule and the chromatographically separated second biomolecule by mass spectrometry to determine the presence or amount of the first biomolecule and the second biomolecule in the sample.
1 more element
Dependencies are inferred from the claim text.